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cDNA Library
A complementary DNA (cDNA) library is a collection of genes for some given organism in which all the introns have been removed. In order to create a cDNA library, we must allow the eukaryotic cell to transcribe the pre-mRNA and then allow RNA processing to take place. This will ensure that all the introns are removed and the exons are spliced together. Once the fully processed mRNA is formed, we can use reverse transcriptase to form the DNA molecule that is complementary to the processed mRNA. This complementary DNA molecule will no longer contain the introns. The double stranded mRNA-cDNA can be separated by heating and then a DNA polymerase can be used on the single-stranded cDNA molecule to form the more stable double stranded cDNA molecule. This process can be repeated with all the genes to form the complete cDNA library for that particular organism. What is the benefit a cDNA library versus a regular gene library? Recall that bacterial cells cannot process mRNA that contain introns. The cDNA library allows us a way to use bacterial cells to process the genes of interest and build a variety of different kinds of eukaryotic proteins.
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